Long oligos Synthesis. Many oligonucleotide synthesis facilities struggle to produce good quality long oligos, and researchers often mistakenly believe that this is due to deficiencies in the purification protocol. This is usually not the case, a flawed synthesis cannot be rescued in the purification step. It is an inescapable fact that the longer the oligonucleotide, the more difficult the.
Long Oligos Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long-oligo synthesis. The techniques we have developed to purify long oligos are unmatched by other suppliers.
While there has been significant demand for long oligos (150 mer and more), the yields in conventional DNA synthesis and the associated side reactions have previously limited the availability of oligonucleotides to lengths 150 nt. Bio-Synthesis's expertise in artificial gene synthesis provides us with the ability to synthesize thousands of single strand synthetic long DNA oligos using the best.Our B-pure Oligonucleotide Synthesis protocol produces custom synthesis of affordable, high-quality long oligos up to 300 bases. For oligonucleotides longer than 80 bases the B-pure protocol is the standard synthesis method.Long RNA oligo synthesis is used in the production of Synthetic long RNA oligos for a variety of applications ranging from understanding the function of newly discovered genes to gene analysis. Long RNA oligos function as positive controls for sensitive molecular diagnostic tools and the development of medical therapeutics. Bio-Synthesis offers.
Oligo synthesis: Why IDT leads the oligo industry Read about the phosphoramidite method of oligonucleotide synthesis that IDT uses in its manufacturing processes. We also highlight the additional measures we take to ensure our customers receive the highest quality oligos and nucleic acid products in the shortest time possible.Read More
Oligo-Flex Synthesis GENEWIZ Oligo-Flex Synthesis offers customized oligos with a wide variety of modifications, at several scales, with HPLC or PAGE purification options. All oligos are synthesized under GENEWIZ’s strict quality control system, with mass spectrometry quality analysis for most oligo types to ensure accuracy and purity. Custom-designed oligos may also be available on request.Read More
Oligo-Flex: Synthesis of custom length oligos (5-150 nt) at 25 - 1000 nmol scales. 5', internal, or 3' modifications are available with HPLC, PAGE, and desalted purification options. Turnaround time starts at 4 business days, depending on scale and modification requirements. Oligos Retained for Sanger: Order an oligo for a Sanger sequencing.Read More
By leveraging our advanced and innovative manufacturing process, Synbio Technologies offers a wide range of oligo synthesis options including standard oligos, long oligos, ultra-high purity oligos, degenerate primers, aptamers, fluorescent probes and oligo pool synthesis, etc. In addition, we also provide high-throughput oligo synthesis to customers with large-scale oligo projects. Synbio.Read More
Technical Resources for Oligonucleotides. The naturally occurring mutations is an event inherent to the chemical synthesis of the oligos and the chances of having one single insertion or deletion in a given oligo of about 30 bases is about 2%. We will be happy to replace any oligo that falls into this category. With regards to the perceived mutations, following DNA synthesis, the completed.Read More
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure ().The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical.Read More
Oligo Modification. over 300 modifications available; Cost-effective alternative modifications for VIC, NED and PET dyes; DNA, RNA 2'0-Me, LNA backbones; Oligos can be modified in several ways by utilizing the active groups of the nucleotide or creating nucleotide analogues. We supply a long list of modifications for your oligos and this list.Read More
Thiol-modified oligos are synthesized by incorporating the thiol group (with C3 or C6 linker) during oligo synthesis at either the 5’- or 3’- end. Thiol-modified oligos can be linked to peptides or enzymes; for example oligo probes labelled with alkaline phosphatase enzyme can be used for in situ hybridization. Thiol-modified oligos can also be used for immobilization on surfaces and for.Read More
The MerMade by Bioautomation is well-established in the Middle East, Africa and other countries where delivery of custom oligos often takes a long time. This machine can be maintained by yourself.Read More
Oligo Synthesis ShineGene has synthesized millions of high-quality oligonucleotides for researchers since it was founded in 2003. The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers.ShineGene offer you an innovative line of custom oligonucleotides for your research.Read More